胚珠培養による数種ブラシカの種間雑種形成に及ぼす母本と置床時の胚珠の齢の影響

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  • Effects of the Species of Female Parent and the Age of Ovules on Interspecific Hybrid Formation in Several Brassicas by Ovule Culture.

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To examine the effects of the species of the female parent and the age of ovules at ovule explantation after cross-pollination on interspecific hybrid formation among broccoli (Brassica oleracea L. var. italica Plen.), Chinese kale (B. oleracea L. var. alboglabra L. H. Bailey), broccoli raab (B. raga L.) and nabana (leafy rape : B. napus L.), an ovule culture was carried out. Ovules were explanted at 5 day intervals from 5 to 40 days after cross-pollination (DAP) and were cultured on MS medium supplemented with 0, 0.1 and 1mg/l of naphthalene acetic acid (NAA) and kinetin.<BR>When ovules were explanted at 25 to 40 DAP, a higher rate of hybrid formation in several combinations of interspecific crossings was observed than in the earlier explantings. The percentage hybrid formation was dependent on selection of the female parent. When broccoli or Chinese kale was used as the female parent and broccoli raab was used as the pollen parent, hybrid plantlets were formed at higher percentages, regardless of the age of ovule at ovule explanting, compared with reciprocal combinations. When nabana or Chinese kale was used as the female parent and broccoli or nabana was used as the pollen parent, respectively, many hybrid plantlets were formed, regardless of the age of ovule at explanting, compared with reciprocal combinations. Higher concentrations of NAA in the medium may have suppressed hybrid formation.

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