Molecular Design of Chemical Probe for Bioimaging of Nitrogen Monooxide

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  • NAGANO Tetsuo
    Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • KOJIMA Hirotatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo

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  • 一酸化窒素のバイオイメージングを目的とした生体プローブの分子設計
  • 1サンカ チッソ ノ バイオイメージング オ モクテキ ト シタ セイタイ プ

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Abstract

The paper introduces development of the molecular probe for bioimaging of nitrogen monoxide (NO). First, we review the biological importance of NO and the NO detection methods. There are several strategies used most widely to detect NO: 1) a chemiluminescence assay based on the reaction of NO with ozone, 2) a spectrophotometric assay which measures the product in the nitrite azo-coupling reaction, 3) an oxidation assay of hemoglobin to methemoglobin by NO, 4) a GC/MS assay for direct detection of NO, 5) electron paramagnetic resonance (ESR) assay for detection of a stable adduct formed in the reactions of NO and certain compounds, 6) electrochemical assays with specific electrodes for NO, 7) radioisotope assay, 8) fluorescence assay using 2, 3-diaminonaphthalene, and 9) chemiluminescence assay with luminol + hydrogen peroxide system. Secondly, we introduce how to develop the novel probes, diaminofluoresceins (DAFs), for detection of NO produced in living cells, tissue cultures or in vivo s ystems. Finally DAFs are applicable for detection of NO produced from induced NO synthase (i-NOS) or constitutive NO synthase (c-NOS). In addition, we review the biological application of 3', 6'-diacetoxy5, 6-diaminospiro [isobenzofuran-1 (3H), 9'-[9H] xanthen] -3-one (DAF-2DA) useful for bioimaging of NO in living cells.

Journal

  • NIPPON KAGAKU KAISHI

    NIPPON KAGAKU KAISHI 1998 (11), 721-729, 1998-11-10

    The Chemical Society of Japan

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