Micro Determination of Albumin Utilizing the Reaction with Iodine

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Other Title
  • ヨウ素との反応を利用した微量アルブミンの定量

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A rapid method using an iodide-selective electrode was proposed for the determination of lactalbumin, egg albumin and bovine serum albumin by potentiometric argentimetric titration of iodide ions, liberated in proportion to the amount of albumin at constant temperature when albumin was treated with an iodine-methanol solution.<BR>The reaction ratios of standard amino acids to iodine, effect of amounts of iodine, effect of methanol, effect of temperature, effect of added sulfuric acid, effect of reaction time, effect of concomitant compounds and precision data of albumin were discussed.<BR>Recommended procedure is as follows. A sample containing 0.05-60 mg lactalbumin, 0.470 mg egg albumin or 0.5-120 mg bovine serum albumin was taken into a 50 ml-titration cell and treated with 10 ml of 50 mmol⋅dm-3 iodine-methanol solution. In addition, 3 ml of 1mol⋅dm-3 sulfuric acid was introduced into sample solution. The volume was adjusted with distilled water to 50 ml which was then titrated potentiometrically with a 0.5-20 mmol⋅dm°C standard silver nitrate solution. The temperature was maintained at 30°C through these procedures.<BR>By addition of sulfuric acid, potential jump of titration curve was enhanced. Lactalbumin (0.05-60 mg), egg albumin (0.4, -70 mg) and bovine serum albumin (0.5-120 mg) were determined with a coefficient of variation of less than 4.8% at 30°C. The calibration curves of lactalbumin, egg albumin and bovine serum albumin were shown by the equations of Y=0.285 X+ O.058 (r= O.999), Y=O.208 X-1-0.022 (r =0.999) and Y = 0.153 X-0.028 (r= 0.999), respectively; Y being the silver nitrate weight added in mg, X albumin weight in mg and r correlation coefficients.

Journal

  • NIPPON KAGAKU KAISHI

    NIPPON KAGAKU KAISHI 1988 (7), 1021-1025, 1988-07-10

    The Chemical Society of Japan

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