Evaluation of Alveolar Bone Resorption and Formation Using Biochemical Markers Specific for Bone Metabolism.

DOI 4 Citations 44 References
  • TSUKADA Eiji
    Department of Periodontology, Asahi University, School of Dentistry

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  • 歯槽骨の吸収ならびに形成時における骨代謝マーカーの変動

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The purpose of this study was to evaluate Deoxy -piridinoline (DPD) and Osteocalcin (OC), which are bone metabolic markers, in alveolar bone turnover. For the in vitro study, medium was recovered after isolated rabbit osteoclasts had been cultured on canine alveolar bone slices for 48 hr to study the relation between alveolar bone resorption and DPD produced in the medium. Experimental periodontitis was induced by silk thread ligation in the gingival sulci of beagle dogs. Gingival crevicular fluid (GCF), urine and serum were collected at 0, 3, 7, 14 and 21 days after ligature. DPD was measured by the ELISA method using monoclonal antibody. Furthermore, to evaluate OC in serum as a marker of alveolar bone formation, blood samples were collected every week after surgical bone defects had been prepared adjacent to a mandibular premolar tooth.<BR>As a result, (1) The level of DPD in the medium increased significantly in co-culture with osteoclasts and bone as compared to controls. (2) In GCF and urine, the DPD level increased dramatically on day 3 but decreased at 14 and 21 days. DPD did not fluctuate in serum during the experimental period. (3) The level of OC in serum increased significantly at 1, 2 and 3 weeks after surgery in the formation of new bone.<BR>These suggest that DPD in GCF and urine and OC in serum may be suitable markers for alveolar bone remodeling in periodontal disease.

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