イヌ歯槽骨のプロテオグリカン

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  • Proteoglycans in Alveolar and Non-Alveolar Regions of Canine Mandibular Bone.

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Proteoglycans in alveolar and non-alveolar regions of canine mandibular bone were extracted and separated by gel-filtration and SDS-polyacrylamide gel electrophoresis. Furthermore, qualitative and quantitative analysis of glycosaminoglycans liberated from these proteoglycans was performed to characterize the proteoglycans in alveolar bone as compared to those in non-alveolar bone. Proteoglycans in bone tissues were successfully extracted under demineralizing conditions achieved in the presence of 0.25M ethylenediaminetetraacetic acid. The amount of proteoglycan in alveolar bone was less than 50% of that in non-alveolar bone. The molecular weights of proteoglycans partially purified by CsC1 centrifugation ranged from 70, 000 to76, 000. It is possible that two different low molecular weight forms exist in bone. The glycosaminoglycans extracted from the proteoglycans consisted mainly of chondroitin sulfate in both alveolar and non-alveolar bones.

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