Potential Application of Molecular Techniques for the Evaluation of Endocrine-disrupting Chemicals in Leachate from Waste Landfill

Ishibashi Hiroshi, Tominaga Nobuaki, Arizono Koji

doi:10.3985/wmr.15.240

Bibliographic Information

Other Title

内分泌撹乱作用を検出する遺伝子レベルのバイオアッセイ
ナイブンピカクランサヨウオケンシュツスルイデンシレベルノバイオアッセイ

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Description

We critically review the current and potential application of molecular techniques in assessing the adverse biological reproductive effects of endocrine-disrupting chemicals (EDCs) in leachate from waste landfills. Various in vitro screening assays have been developed to evaluate EDCs that may bind to sex hormone receptors. Screening assays such as the receptor-binding assay to check the direct binding ability of a chemical with the receptor and the use of cultured cells such as MCF-7 cells and yeast cells in which the receptor gene is inserted are frequently used. As for in vivo assays, plasma and/or hepatic vitellogenin induction assays to determine estrogenic activity have been used in fish and amphibians. In addition, molecular techniques, such as real-time polymerase chain reaction (PCR) and cDNA-microarray, are considered to be useful tools for risk assessment of animals and humans exposed to leachate from waste landfills. This review provides a link between traditional single-gene biomarker studies and the emerging field of eco-toxicogenomics, demonstrating the utility of microarray studies on environmentally sampled, non-model organisms.

Journal

Waste Management Research

Waste Management Research 15 (5), 240-246, 2004

Japan Society of Material Cycles and Waste Management

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Details 詳細情報について

CRID: 1390282679392500608

NII Article ID: 10013394466

NII Book ID: AN10414674

DOI: 10.3985/wmr.15.240

ISSN: 18838960; 09170855

NDL BIB ID: 7127917

Web Site: http://id.ndl.go.jp/bib/7127917; https://ndlsearch.ndl.go.jp/books/R000000004-I7127917

Text Lang: ja

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