N,N'-Bis(2-chloroethyl)- N-nitrosourea (BCNU)-induced Apoptosis of Neural Progenitor Cells in the Developing Fetal Rat Brain
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- Yamaguchi Tsuyoshi
- Bozo Research Center Inc. Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences
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- Kanemitsu Hiroyuki
- Bozo Research Center Inc.
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- Yamamoto Satoshi
- Bozo Research Center Inc.
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- Komatsu Masahiko
- Bozo Research Center Inc.
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- Uemura Hiroyuki
- Bozo Research Center Inc.
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- Tamura Kazutoshi
- Bozo Research Center Inc.
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- Shirai Tomoyuki
- Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences
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N,N'-bis(2-chloroethyl)-N-nitrosourea (BCNU) is one of the major drugs used in chemotherapy against malignant gliomas due to its effects, such as induction of bifunctional alkylation of DNA and formation of interstrand DNA cross-linkages, and induces cortical malformations in the fetal and neonatal rat brain. In this study, pregnant rats were treated with 7.5 mg/kg of BCNU on gestational day 13 (GD 13), and their fetuses were collected from 12 to 72 hours after BCNU treatment in order to examine the timecourses of morphological and immunohistochemical changes in neural progenitor cells in the developing brain. The number of pyknotic cells in the telencephalon peaked at 24 h and then gradually decreased until 72 h. The majority of these pyknotic cells were positive for cleaved caspase-3, a key executioner of apoptosis. The pyknotic cells showed the ultrastructural characteristics of apoptosis. The number of p53-positive cells began to increase prior to the appearance of apoptotic cells and p21-positive cells. The number of phosphorylated-histone H3-positive cells (mitotic cells) decreased from 24 to 36 h. The number of Iba1-positive cells (microglial cells) in the telencephalon increased from 12 to 48 h. These results suggest that BCNU induces p53-dependent apoptosis and reduces proliferative activity, resulting in reduction of the weight of the telencephalon and the thickness of the telencephalic wall in the fetal brain. This study will help to clarify the mechanisms of BCNU-induced fetal brain toxicity.<br>
収録刊行物
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- Journal of Toxicologic Pathology
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Journal of Toxicologic Pathology 23 (1), 25-30, 2010
日本毒性病理学会
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詳細情報 詳細情報について
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- CRID
- 1390282679393182464
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- NII論文ID
- 10030978555
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- NII書誌ID
- AN10232280
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- ISSN
- 1881915X
- 09149198
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- NDL書誌ID
- 10647689
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可