Cadystins((.GAMMA.-EC)nG) Synthesis Ability of Carboxypeptidase(CPase).

DOI Web Site 1 Citations 14 References

Bibliographic Information

Other Title
  • カルボキシペプチダーゼのカディスチン[(γ-EC)<SUB>n</SUB>G]合成能
  • Cadystins[(γ-EC)nG]Synthesis Ability of Carboxypeptidase(CPase)
  • Cadystins ガンマーEC nG Synthesis Ability of Carboxypeptidase CPase

Search this article

Abstract

The authors characterized a enzyme that is responsible for (γ-EC)nG synthesis in rice root and it was confirmed that at least one of the (γ-EC)nG synthases in the rice root may be carboxypeptidase (CPase). We examined the ability of CPases in vitro to (γ-EC)nG synthesis and to confirm that CPase in rice plant have the ability to synthesis (γ-EC)nG. The structural gene of the rice CPase was ligated to the expression vector, transformed into E. coli and expressed. All of the CPase (CPase W, Y, and A) were examined synthesized having ability to (γ-EC)2G in vitro. On the contrary, none of the aminopeptidase synthesize it examined in this study. The specific inhibitor of CPase inhibited (γ-EC)2G synthesis. The optimum pHs of the synthesis with each CPase are the same as optimum pHs for the hydrolytic activity of the respective enzymes. The (γ-EC)nG synthesis activity was increased through CPase gene transformation E. coli. These results suggest that at least one of the (γ-EC)nG synthases is CPase.

Journal

  • ENVIRONMENTAL SCIENCE

    ENVIRONMENTAL SCIENCE 12 (4), 393-398, 1999

    SOCIETY OF ENVIRONMENTAL SCIENCE, JAPAN

Citations (1)*help

See more

References(14)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top