89. Mode of action and cell response of jasmonate glucosides
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- Nakamura Yoko
- Grad. School of Sci., Tohoku Univ.
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- Hamamoto Shin
- Grad. School of Engineering., Tohoku Univ.
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- Inomata Sho
- Grad. School of Sci., Tohoku Univ.
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- Mithofer Axel
- Max-Planck-Institute for Chemical Ecology
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- Boland Wilhelm
- Max-Planck-Institute for Chemical Ecology
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- Uozumi Nobuyuki
- Grad. School of Engineering., Tohoku Univ.
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- Ueda Minoru
- Grad. School of Sci., Tohoku Univ.
Bibliographic Information
- Other Title
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- 89.ジャスモン酸配糖体の作用機構と細胞応答(口頭発表)
Abstract
Samanea plants close their leaves in the evening, as if to sleep, and open them in the morning according to the circadian rhythm. Potassium β-D-glucopyranosyl-12-hydroxyjasmonate (1) was isolated as leaf-closing factor (LCF) of S. saman. We developed molecular probes consisting of modified LCF1 in order to identify its mode of action. We have already demonstrated that a specific binding protein is involved in the motor cell of S. saman. We synthesized natural-type photoaffinity probe and biologically inactive enantiomer-type probe. We utilized them for photoaffinity labeling of the target protein for LCF1. By using protoplasts of motor cell, we found membrane protein of 38kDa which strictly recognizes the stereochemistry of 1, and it is highly likely that the protein is the target protein for LCF 1. Recently, we observed that LCF shrank motor cell protoplasts prepared from S. saman. And comparing the results of several bioassay using glucosyl jasmonate-type LCF and jasmonic acid, it is also interesting that the mode of action of LCF is completely different with that of jasmonate.
Journal
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- The Janapese Society for Chemical Regulation of Plants, Abstract
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The Janapese Society for Chemical Regulation of Plants, Abstract 44 (0), 103-, 2009
The Janapese Society for Chemical Regulation of Plants
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Details 詳細情報について
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- CRID
- 1390282679399120000
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- NII Article ID
- 110007468349
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- ISSN
- 21896313
- 09191887
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed