INVOLVEMENT OF COTYLEDON AND GIBBERELLIN IN THE TISSUE-REUNION PROCESS OF CORTEX IN THE CUT HYPOCOTYL OF CUCUMBER AND TOMATO
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- Asahina Masashi
- Institute of Biological Sciences, University of Tsukuba
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- Iwai Hiroaki
- Institute of Biological Sciences, University of Tsukuba
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- Kikuchi Akira
- Institute of Biological Sciences, University of Tsukuba
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- Yamaguchi Shinjiro
- Plant Science Center, RIKEN
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- Kamiya Yuji
- Plant Science Center, RIKEN
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- Kamada Hiroshi
- Institute of Biological Sciences, University of Tsukuba
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- Satoh Shinobu
- Institute of Biological Sciences, University of Tsukuba
Bibliographic Information
- Other Title
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- 15 キュウリ・トマト切断胚軸における皮層の組織癒合に対する子葉およびジベレリンの関与
Abstract
Cucumber hypocotyls were cut in half transversely, and morphological and histochemical analyses of the process of tissue-reunion in the cortex were performed. Cell division in the cortex commenced 3 days after cutting, and the cortex was nearly fully united within 7 days. DAPI- staining and BrdU-labeling experiments indicate that nuclear DNA synthesis occurred during this process. A specific accumulation of pectic substances was observed in the cell wall of attached cells in the reunion region of the cortex. Cell division in the tissue-reunion process in the cortex was strongly inhibited by removal of the cotyledon, and this inhibition was reversed by the application of gibberellin (10^<-4> M GA_3), but not auxin (10^<-4> MIAA), to the tip of the cotyledon-excised plant. Furthermore, cell division in the cortex was inhibited by treatment of the cotyledon with 10^<-4>M uniconazole-P, and this inhibition was also reversed by simultaneous application of GA. In contrast, removing the shoot apex did not inhibit tissue-reunion, and normal cortex tissue-reunion was observed when 10^<-4>M TIBA was applied to the upper hypocotyl above the position of the cut. Furthermore, similar phenomena were observed in tomato seedlings, and cell division in the tissue-reunion process did not occur in cut hypocotyls of a tomato GA-deficient mutant (gib-1). These results suggest novel functions for GA in cell division during the process of tissue-reunion in the cortex, as well as involvement of the cotyledon in the production of GA.
Journal
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- The Janapese Society for Chemical Regulation of Plants, Abstract
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The Janapese Society for Chemical Regulation of Plants, Abstract 36 (0), 41-42, 2001
The Janapese Society for Chemical Regulation of Plants
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Details 詳細情報について
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- CRID
- 1390282679401569152
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- NII Article ID
- 110001853790
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- ISSN
- 21896313
- 09191887
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed