書誌事項
- タイトル別名
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- Evaluation of a Rapid Test for Determination of Most Probable Number of Viable <i>Vibrio parahaemolyticus</i> by Using Etidium Monoazide
- Ethidium monoazide オ モチイタ チョウエン ビブリオ セイキン ノ ジンソク テイリョウホウ ニ カンスル ケントウ
- Evaluation of a Rapid Test for Determination of Most Probable Number of Viable Vibrio parahaemolyticus by Using Etidium Monoazide
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抄録
Ethidium monoazide (EMA) has been used for selective polymerase chain reaction (PCR) amplification of DNA extracted from viable bacterial cells. However, its use is very limited; further, it is less frequently applied for quantitative purposes, for example, bacterial counting. In this study, we evaluated the most probable number (MPN)-PCR method using the species-specific toxR as the target gene after EMA treatment, for rapid quantitative determination of viable Vibrio parahaemolyticus (EMA+MPN-PCR). In addition, to avoid false-negative results attributable to PCR inhibition owing to carryover contamination, we added the positive-control template DNA (PCT) to each reaction tube. The optimal concentration of EMA for V. parahaemolyticus cells was determined to be 5 μg/ml in the preliminary experiments; subsequnently, the effectiveness of EMA was confirmed using simulated food samples spiked with viable or dead V. parahaemolyticus cells. The results of the experiments with 38 seafood and 8 seawater samples were as follows: MPN values determined using EMA+MPN-PCR were almost the same as or higher than those determined using the official method. Moreover, with the EMA+MPN-PCR method, other Vibrio species (e.g., Vibrio harveyi) could be specifically distinguished from V. parahaemolyticus. EMA+MPN-PCR was shown to be a rapid and sensitive technique for counting viable V. parahaemolyticus in food and environmental samples.
収録刊行物
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- 日本食品微生物学会雑誌
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日本食品微生物学会雑誌 28 (1), 21-28, 2011
日本食品微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679402156800
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- NII論文ID
- 130004696645
- 10028189305
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- NII書誌ID
- AN10552871
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- COI
- 1:CAS:528:DC%2BC3MXlslOnt7o%3D
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- ISSN
- 18825982
- 13408267
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- NDL書誌ID
- 11057789
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可