Evaluation of Bioluminescent Enzyme Immunoassay: BLEIA to Detect Norovirus in Environmental Swabs

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  • 環境中のノロウイルスの拭き取り検査における生物発光酵素免疫測定法（BLEIA法）の検討
  • カンキョウ チュウ ノ ノロウイルス ノ フキトリ ケンサ ニ オケル セイブツ ハッコウ コウソ メンエキ ソクテイホウ(BLEIAホウ)ノ ケントウ

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Abstract

We swabbed surfaces to detect the presence of Norovirus (NV) using bioluminescent enzyme immunoassay (BLEIA) and to assess the accuracy and usefulness of this method compared with the standard method of real-time PCR. All samples obtained were assessed via both methods. We determined that BLEIA was not only accurate and effective but also cheaper than real-time PCR. Moreover, the simultaneous processing of a large number of samples was easier using BLEIA. Therefore, we recommend the use of BLEIA to rapidly detect NV on a plethora of surfaces.

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