DNA Fingerprinting of Cultivated Laver Porphyra yezoensis and P.tenera with Oligonucleotide Probes,and Its Application to Cultivar Discrimination
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- Mizukami Yuzuru
- Resource Enhancement Division, Seikai National Fisheries Research Institute
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- Okauchi Masanori
- Resource Enhancement Division, Seikai National Fisheries Research Institute
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- Kito Hitoshi
- Shimonoseki University of Fisheries
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- Kobayashi Masahiro
- Resource Enhancement Division, Seikai National Fisheries Research Institute
書誌事項
- タイトル別名
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- DNA Fingerprinting of Cultivated Laver <i>Porphyra yezoensis</i> and <i>P. tenera</i> with Oligonucleotide Probes, and Its Application to Cultivar Discrimination
- DNA Fingerprinting of Cultivated Laver
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抄録
Synthetic oligonucleotides were used to identify motifs of simple repetitive sequences in cultivated laver (Porphyra yezoensis and P. tenera) genome by Southernhybridization.Three oligonucleotides, (CAC)6, (GT)8 and (GGAT)4, hybridized intensely with genomic DNA, suggesting that these simple repetitive sequences were abundant and highly dispersed in the laver genome. Withthese oligonucleotides as probes, DNA fingerprinting was examined to distinguish cultivars of the laver. When genomic DNA from six cultivars were digested with Hae III or Afa I, and hybridized with oligonucleotide probes, hybridization patterns appeared to be specific for individual cultivars. In addition, these fingerprints seemed to be similar duringseveral years. These results indicate that cultivars of laver can be distinguished from each other on the basis of the polymorphic patterns of the repetitive sequences.
収録刊行物
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- Fisheries science
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Fisheries science 62 (2), 173-177, 1996
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390282679404674304
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- NII論文ID
- 130003902893
- 10004865304
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 3951186
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可