DNA Fingerprinting of Cultivated Laver Porphyra yezoensis and P.tenera with Oligonucleotide Probes,and Its Application to Cultivar Discrimination

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  • DNA Fingerprinting of Cultivated Laver <i>Porphyra yezoensis</i> and <i>P. tenera</i> with Oligonucleotide Probes, and Its Application to Cultivar Discrimination
  • DNA Fingerprinting of Cultivated Laver

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Synthetic oligonucleotides were used to identify motifs of simple repetitive sequences in cultivated laver (Porphyra yezoensis and P. tenera) genome by Southernhybridization.Three oligonucleotides, (CAC)6, (GT)8 and (GGAT)4, hybridized intensely with genomic DNA, suggesting that these simple repetitive sequences were abundant and highly dispersed in the laver genome. Withthese oligonucleotides as probes, DNA fingerprinting was examined to distinguish cultivars of the laver. When genomic DNA from six cultivars were digested with Hae III or Afa I, and hybridized with oligonucleotide probes, hybridization patterns appeared to be specific for individual cultivars. In addition, these fingerprints seemed to be similar duringseveral years. These results indicate that cultivars of laver can be distinguished from each other on the basis of the polymorphic patterns of the repetitive sequences.

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