Differential expressin of two gonadotropin(GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica
-
- SUETAKE HIROAKI
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- OKUBO KATAAKI
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- SATO NARUMI
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- YOSHIURA YASUTOSHI
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- SUZUKI YUZURU
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- AIDA KATSUMI
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
書誌事項
- タイトル別名
-
- Differential expression of two gonadotropin (GTH) .BETA. subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica.
- Differential expressin of two gonadotropin GTH ベータ subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica
この論文をさがす
説明
Repeated injection of salmon gonadotropin (sGTH) can induce ovarian maturation in the female Japanese eel Anguilla japonica. The participation of endogenous GTH in this process remains unclear. The steady-state mRNA levels of pituitary GTH Iβ and GTH IIβ subunits in individual fish was investigated using a real-time quantitative reverse transcription-polymerase chain reaction assay based on the TaqManTM fluorogenic detection system. Pituitary GTH Iβ mRNA levels were high when the fish were immature, particularly before being injected with sGTH, but levels decreased with the progression of ovarian development and remained very low from the late vitellogenic stage until postovulation. In contrast, mRNA levels of GTH IIβ were very low until the previtellogenic stage after injection with sGTH, and increased markedly in accordance with ovarian maturation and ovulation. These results suggest that sGTH injection inhibits GTH I synthesis, whereas it induces ovarian maturation and stimulates GTH II synthesis. Thus, the differential expression of GTH Iβ and GTH IIβ genes during artificial maturation suggests that the two GTH are synthesized sequentially and have separate functional roles relating to reproductive events in the Japanese eel.
収録刊行物
-
- Fisheries science
-
Fisheries science 68 (2), 290-298, 2002
公益社団法人 日本水産学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679404813568
-
- NII論文ID
- 130003742436
- 10008272998
-
- NII書誌ID
- AA10993718
-
- ISSN
- 14442906
- 09199268
-
- NDL書誌ID
- 6151841
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
- OpenAIRE
-
- 抄録ライセンスフラグ
- 使用不可