Chimeric Fish Produced by Exchanging Upper Parts of Blastoderms in Goldfish Blastulae
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- Yamaha Etsuro
- Nanae Fish Culture Experimental Station, Faculty of Fisheries, Hokkaido University Laboratory of Breeding Sciences, Faculty of Fisheries, Hokkaido University
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- Mizuno Toshiro
- Department of Molecular Biology, School of Science, Nagoya University
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- Hasebe Yutaka
- Laboratory of Breeding Sciences, Faculty of Fisheries, Hokkaido University
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- Yamazaki Fumio
- Laboratory of Breeding Sciences, Faculty of Fisheries, Hokkaido University
書誌事項
- タイトル別名
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- Chimeric Fish Produced by Exchanging Up
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Chimeric fish were produced by exchanging the upper halves of blastoderms at theblastula stage between goldfish-common carp hybrids with pigmented scales and goldfish with clearscales, or between goldfish with clear scales and triploid crucian carp with pigmented scales. Blastoderm transplantation was carried out in Ringer's solution containing 1.6% albumen within one minute after isolation of the blastoderm. In the absence of albumen, the transplantation was not successful. The larvae and young fish were confirmed to be chimeric fish, as transplanted blastoderm cells could be detected histologically and as the fish exhibited phenotypes typical of both fish used to produce the chimeras. Blastomere mixing between the upper exchanged and lower halves of blastoderms occurred but notto a great extent, and transplanted cells from the upper halves of blastoderms labeled with biotin were mainly distributed in the head region.
収録刊行物
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- Fisheries science
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Fisheries science 63 (4), 514-519, 1997
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390282679405470720
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- NII論文ID
- 10004870606
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- NII書誌ID
- AA10993718
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- COI
- 1:CAS:528:DyaK2sXls1ymsrs%3D
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- NDL書誌ID
- 4277895
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可