Long-term Cell Culture of Resident Peritoneal Macrophages from Red Sea Bream Pagrus major
-
- Watanabe Tasuku
- Laboratory of Fish Pathology, Department of Veterinary Sciences, Nihon University Laboratory of Fish Pathology, Faculty of Fisheries Sciences, Kitasato University
-
- Shoho Tetsuya
- Laboratory of Fish Pathology, Department of Veterinary Sciences, Nihon University
-
- Ohta Hiroshi
- Laboratory of Fish Pathology, Department of Veterinary Sciences, Nihon University
-
- Kubo Naoya
- Laboratory of Fish Pathology, Department of Veterinary Sciences, Nihon University
-
- Kono Michiko
- Fisheries Research Laboratory, the University of Tokyo
-
- Furukawa Kiyoshi
- Department of Fisheries Sciences, Faculty of Agriculture, the University of Tokyo
書誌事項
- タイトル別名
-
- Long-term Cell Culture of Resident Peritoneal Macrophages from Red Sea Bream <i>Pagrus major</i>
- Long-term Cell Culture of Resident Peri
この論文をさがす
抄録
We tried to culture the resident peritoneal macrophage (RPM) of red sea bream (RBS) and obtained transferable cultured cells, when cultivated using RPMI1640 supplemented with 0.4% NaCl and 20% fetal bovine serum at 30°C in 5% CO2. The cells had phagocytic activity against Latex beads and had acid phosphatase and nonspecific esterase activities, both of which are principal characteristics of RBS RPM. Electron microscopically, the cells had slender microvilli, micropinocytic vesicle-like vacuoles, and microfibrils, similar to RSB RPM. The cells had receptors for RSB IgM on the cell surface. The cultured cells migrated to 10-8M N-formylmethionyl leucocyl phenylalanine (FMLP) and the supernatants of the cultured cells. The results suggest that resident peritoneal macrophages produce and release chemotactic factor-like substance (s) in the abdominal cavity.
収録刊行物
-
- Fisheries science
-
Fisheries science 63 (6), 862-866, 1997
公益社団法人 日本水産学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679405657088
-
- NII論文ID
- 130003903128
- 10004871985
-
- NII書誌ID
- AA10993718
-
- NDL書誌ID
- 4371607
-
- ISSN
- 09199268
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可