Differential Expression of mRNAs in the Marine Diatom Chaetoceros compressum Exposed to High Temperatures

DOI Web Site Web Site 被引用文献1件 参考文献27件
  • Kinoshita Shigeharu
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Kikuchi Kiyoshi
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Yamada Hiroshi
    Central Laboratory Marine Ecology Research Institute
  • Hara Takeya
    Central Laboratory Marine Ecology Research Institute
  • Itoh Yasuo
    Central Laboratory Marine Ecology Research Institute
  • Kinoshita Hideaki
    Central Laboratory Marine Ecology Research Institute
  • Watabe Shugo
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

書誌事項

タイトル別名
  • Differential Expression of mRNAs in the Marine Diatom <i>Chaetoceros compressum</i> Exposed to High Temperatures
  • Differential Expression of mRNA s in th

この論文をさがす

抄録

We investigated mRNA expression patterns in the laboratory-reared diatom Chaetoceros compressum under temperature stress conditions. The diatom incubated at 30°C for 15min, showing 43% growth rate in a subsequent culture at 20°C for 2 days, were analyzed by mRNA arbitrarily primed reverse transcription-polymerase chain reaction (RAP RT-PCR) in comparison with control cells incubated at 20°C throughout. The heat stress at 30°C induced expression changes in 7 cDNAs, one of which showed 37% identity to a homologous region of the Bacteroides fragilis glutamine synthetase gene. This stress on the diatom resulted in 2.5-fold increase in its mRNA levels. Our results demonstrated that the gene (s) related to this cDNA is expressed in response to heat stress for the diatom, suggesting its possible applications to monitoring marine environments.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (27)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ