Imaging of Structural Changes in Endothelial Cells and Thrombus Formation at the Site of FeCl<sub>3</sub>-Induced Injuries in Mice Cremasteric Arteries

DOI Web Site PubMed 9 Citations 36 References Open Access
  • Kawamura Yota
    Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.
  • Takahari Yoko
    Teaching and Research Support Center, Tokai University School of Medicine.
  • Tamura Noriko
    Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.
  • Eguchi Yu
    Department of Physiology, Tokai University School of Medicine.
  • Urano Tetsuya
    Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.
  • Ishida Hideyuki
    Department of Physiology, Tokai University School of Medicine.
  • Goto Shinya
    Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.

Bibliographic Information

Other Title
  • Imaging of structural changes in endothelial cells and thrombus formation at the site of FeCl3-induced injuries in mice cremasteric arteries

Search this article

Description

Aim: We investigated thrombus formation at the site of functional injury to endothelial cells by FeCl3.<BR>Methods: After preparation of cremasteric arteries of mice, controlled endothelial injury was induced by application of FeCl3. Endothelial cells were rendered fluorescent by addition of FITC (fluorescein isothiocyanate)-labeled isolectin B4. Circulating platelets and leukocytes were made fluorescent by rhodamine 6G. Three-dimensional (3D) growth of thrombi was visualized in real time. Effects of aspirin and clopidogrel pre-treatments on the growth of thrombi were investigated in vivo as well as in an ex vivo flow chamber system.<BR>Results: Endothelial cells were tightly bound to each other to protect local thrombus formation. Platelets started to adhere to endothelial cells when FeCl3 was applied. Three-dimensional growth of thrombi, which takes 10.6±7.5 minutes for complete occlusion in control, can be visualized with our imaging system. Aspirin pre-treatment at the dose tested did not influence either endothelial injury or platelet thrombus growth, while clopidogrel pretreatment significantly inhibited 3D growth and prolonged occlusion time up to 64.6±25.3 minutes (100 mg/kg). A similar inhibiting effect of clopidogrel was reproduced in ex vivo flow chamber experiments.<BR>Conclusions: We have developed an in vivo system to detect thrombus formation after functional damage to the endothelium.

Journal

Citations (9)*help

See more

References(36)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top