Response of osteoblasts and osteoclasts in regenerating scales to gravity loading

DOI Web Site 被引用文献21件 参考文献53件
  • Suzuki Nobuo
    Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa 927-0553, Japan
  • Kitamura Kei-Ichiro
    Department of Clinical Laboratory Science, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Kodatsuno, Ishikawa 920-0942, Japan
  • Omori Katsunori
    Japan Aerospace Exploration Agency, Tsukuba, Ibaraki 305-8505, Japan
  • Nemoto Tetsu
    Department of Clinical Laboratory Science, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Kodatsuno, Ishikawa 920-0942, Japan
  • Satoh Yusuke
    Department of Laboratory Sciences, School of Health Sciences, Kanazawa University, Kodatsuno, Ishikawa 920-0942, Japan
  • J. Tabata Makoto
    Biostructural Science, Department of Hard Tissue Engineering, Division of Bio-Matrix, Graduate School of Tokyo Medical and Dental University, Bukyo-ku, Tokyo 113-8549, Japan
  • Ikegame Mika
    Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Okayama 700-8525, Japan
  • Yamamoto Toshio
    Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Okayama 700-8525, Japan
  • Ijiri Kenichi
    Radioisotope Center, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
  • Furusawa Yukihiro
    Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194, Japan
  • Kondo Takashi
    Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194, Japan
  • Takasaki Ichiro
    Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani, Toyama 930-0194, Japan
  • Tabuchi Yoshiaki
    Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani, Toyama 930-0194, Japan
  • Wada Shigehito
    Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Toyama, Sugitani, Toyama 930-0194, Japan
  • Shimizu Nobuaki
    Institute of Nature and Environmental Technology, Kanazawa University, Kakuma, Ishikawa 920-1192, Japan
  • Sasayama Yuichi
    Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa 927-0553, Japan
  • Endo Masato
    Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan
  • Takeuchi Toshio
    Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan
  • Nara Masayuki
    Department of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827, Japan
  • Somei Masanori
    Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Ishikawa 920-1192, Japan
  • Maruyama Yusuke
    Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827, Japan
  • Hayakawa Kazuichi
    Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma, Ishikawa 920-1192, Japan
  • Shimazu Toru
    Japan Space Forum, Department of Science and Applications, Chiyoda-ku, Tokyo 100-0004, Japan
  • Shigeto Yuko
    Japan Aerospace Exploration Agency, Tsukuba, Ibaraki 305-8505, Japan
  • Yano Sachiko
    Japan Aerospace Exploration Agency, Tsukuba, Ibaraki 305-8505, Japan
  • Hattori Atsuhiko
    Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827, Japan

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It is known that the teleost scale regenerates after being removed. We previously reported that the osteogenesis in regenerating scales was very similar to that in calvarial bone, which suggests that regenerating scale can be used as a model for osteogenesis to analyze the interaction between osteoblasts and osteoclasts. In the present study, we developed an assay system using regenerating scales by modifying our assay system with normal scales. The weight of one regenerating scale cannot be measured precisely because the rate of calcification in regenerating scales is lower than that in normal scales. In regenerating scales, thus, the respective marker enzyme activity, i.e., alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts, was normalized by the surface area (mm2) of each goldfish scale. With this modified method, we were able to detect ALP and TRAP activities with no variation in the lines of regenerating scales. In addition, we found that the ALP activity in the regenerating scales significantly increased under 3G acceleration loading by vibration, while the TRAP activity in the loaded regenerating scales significantly decreased. We strongly believe that the regenerating scale is a good material for the analysis of bone metabolism under different gravity environments.

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