環境水からのＬｏｏｐａｍｐレジオネラ検出試薬キットを用いたＬｅｇｉｏｎｅｌｌａ属菌の検出

雑賀 威, 舘脇 光弘, 金山 明子, 小林 寅哲

doi:10.11550/jsei1986.22.75

書誌事項

タイトル別名

Detection of Legionella Species in Environmental Water Samples by Loopamp Legionella Detection Reagents Kit
カンキョウスイカラノ Loopamp レジオネラケンシュツシヤクキットオモチイタ Legionellaゾクキンノケンシュツ

この論文をさがす

説明

Detection of Legionella species using the loop-mediated isothermal amplification (LAMP) method was investigated with the “Loopamp Legionella detection reagents kit E” (Eiken Chemical Co., Ltd.). The detection limit of the LAMP method was 3.7×10¹ CFU/test, approximately equivalent to the limit claimed by the instructions (60 CFU/test). The LAMP method detected positive reactions for 17 of 100 Legionella samples which gave negative reactions by the culture method, indicating that the sensitivity of the LAMP method was higher than the culture method. No false positive results were obtained in the screening of 5 organisms other than Legionella species. All visual results were equal to those obtained using a turbidity meter. The LAMP method can specifically detect Legionella species at levels lower than the detection limit of the conventional culture method in a short time, so is useful for the prevention of nosocomial Legionella infection.

収録刊行物

環境感染

環境感染 22 (2), 75-78, 2007

日本環境感染学会

キーワード

詳細情報詳細情報について

CRID: 1390282679414653312

NII論文ID: 10024358116

NII書誌ID: AN1019475X

DOI: 10.11550/jsei1986.22.75

ISSN: 18842429; 09183337

NDL書誌ID: 8883685

Web Site: http://id.ndl.go.jp/bib/8883685; https://ndlsearch.ndl.go.jp/books/R000000004-I8883685; https://search.jamas.or.jp/link/ui/2007323008

本文言語コード: ja

データソース種別

JaLC
NDLサーチ
CiNii Articles

抄録ライセンスフラグ: 使用不可

書き出し

問題の指摘