Standing Periods at Room Temperature before the Onset of Cooling Procedure are Unnecessary to Well Accomplish the Sperm Cryopreservation in Okinawa Native Pig, Agu
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- YOSHIMOTO Teppei
- <I>Faculty of Agriculture, University of the Ryukyus</I>
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- NAKAMURA Satoshi
- <I>Okinawa Prefectural Livestock Research Center</I>
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- TOKESHI Isao
- <I>Faculty of Agriculture, University of the Ryukyus</I>
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- NAKADA Tadashi
- <I>Faculty of Agriculture, University of the Ryukyus</I>
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- TATEMOTO Hideki
- <I>Faculty of Agriculture, University of the Ryukyus</I>
Bibliographic Information
- Other Title
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- 琉球在来豚アグー精子における冷却処理前の室温放置が凍結融解後の精子性状に及ぼす影響
Abstract
The technical establishment of boar sperm cryopreservation is indispensable for constantly supplying fine spermatozoa to effectively breed rare Okinawa native pig, Agu. Hence, the present study was carried out to determine whether the standing periods of spermatozoa before the onset of cooling procedure had effects on its freezability in association with the resistance to chilling injury, assessed by the integrity of plasmalemma, the acrosomal proteolytic activity, the motile patterns, and the in vitro fertilizing capacity. Ejaculated spermatozoa from four Agu and two Landrace boars were washed by centrifugation within 10 min after collection, suspended in Beltsville thawing solution, and then allowed to stand for 0, 1 or 2 hour at room temperature until dilution with BF5 freezing solution. In Agu, the elongation of standing periods before cooling caused significant (p<0.05) decreases in the integrity of plasmalemma, the proteolytic activity of acrosomal contents, and the rate of motile cells and progressive motility in frozen-thawed spermatozoa. Moreover, the post-thawed spermatozoa without the standing period possessed the highest level of the penetrability to in vitro matured oocytes. However, the influences of the standing periods upon the freezability were not found in Landrace spermatozoa as Agu spermatozoa. Therefore, it is concluded that the freezing method without the standing periods before the onset of cooling procedure is appropriate for cryopreservation of ejaculated spermatozoa in Agu.
Journal
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- The West Japan Journal of Animal Science
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The West Japan Journal of Animal Science 50 35-42, 2007
Warm Regional Society of Animal Science, Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679417928448
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- NII Article ID
- 130004185290
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- ISSN
- 18846394
- 09143459
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed