Effect of Human Fibroblast-Derived Interleukin-6 on Platelet Levels in Normal Mice

DOI Open Access
  • ASAMI Tetsuya
    Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd.
  • KUSUYAMA Takeshi
    Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd.
  • NODA Kouichi
    Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd.
  • OGINO Hiromi
    Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd.
  • HORISAWA Yoshifumi
    Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd.
  • ISAJI Mitsuko
    Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd.
  • OHTA Hidemi
    Pharmaceutical Laboratory, Mochida Pharmaceutical Co., Ltd.
  • TANAKA Ryo
    Pharmaceutical Laboratory, Mochida Pharmaceutical Co., Ltd.

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Other Title
  • ヒト正常二倍体線維芽細胞由来天然型ヒトインターロイキン6の正常マウスにおける血小板増加作用

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Description

Purified natural human fibroblast-derived interleukin-6 (nhlL-6) was obtained from a culture of a normal human fibroblast cell strain, and its effect on the platelet number in normal mice was examined.<br>The platelet number in female BDF1 mice given subcutaneously 10, 50, or 250μg/kg of nhlL-6 for 5 consecutive days increased significantly in a dose-dependent manner, and the respective platelet counts were 131.4±3.7×104 (119%), 139.8±3.2×104 (127%), and 159.4±4.2×104/μl (144%). The count in control mice was 110.4±2.6×104/μl (100%). The significant increase in megakaryocyte number in the spleens of the mice receiving 250μg/kg was also observed.<br>On the other hand, a substantial decrease in total bile acid in plasma and a remarkable increase in plasma immunosuppressive acidic protein (IAP), an acute phase protein, were found, and moderate but significant changes in parameters such as hemoglobin, hematocrit, plasma amylase, plasma alkaline phosphatase, plasma total cholesterol, and plasma free cholesterol were also observed.

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