A Method for Routine Analysis of Urinary 4,4'-methylenebis (2-chloroaniline) by Gas Chromatography-Electron Capture Detection

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  • Takeuchi Akito
    Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association
  • Namera Akira
    Department of Forensic Medicine, Institute of Biomedical and Health Sciences, Hiroshima University
  • Sakui Norihiro
    Agilent Technologies
  • Yamamuro Kenji
    Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association
  • Kawai Toshio
    Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association
  • Sumino Kimiaki
    Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association

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  • A Method for Routine Analysis of Urinary 4,4′-methylenebis (2-chloroaniline) by Gas Chromatography-Electron Capture Detection
  • A Method for Routine Analysis of Urinary 4,4′‐methylenebis (2‐chloroaniline) by Gas Chromatography‐Electron Capture Detection

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Objectives: The purpose of this study was to develop a simple and cost-effective method for the determination of urinary 4,4′-methylenebis (2-chloroaniline) (MBOCA) by gas chromatography-electron capture detection (GC-ECD) for biological monitoring of occupational exposure to MBOCA. Methods: MBOCA was prepared by liquid-liquid extraction after alkaline hydrolysis, derivatized with N-methyl-bis (trifluoroacetamide) and then analyzed using GC-ECD. The proposed method was validated in accordance with the US Food and Drug Administration guidance. Results: The calibration curve showed linearity in the range 1–100 µg/l, with a correlation coefficient of >0.999. The limits of detection and quantification were 0.3 µg/l and 1 µg/l, respectively. The recovery was 94–99%. Intraday accuracy, expressed as the deviation from the nominal value, was 90.5–100.3%, and intraday precision, expressed as the relative standard deviation, was 0.3–2.4%. Interday accuracy and precision were 87.8–100.2% and 0.3–4.1%, respectively. Conclusions: The proposed method is a simple and cost-effective method suitable for routine analyses and could be useful for biological monitoring of occupational exposure to MBOCA.(J Occup Health 2014; 56: 347-350)

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