Ultraviolet Action Spectrum for Cell Killing of Primary Porcine Lens Epithelial Cells

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  • Okuno Tsutomu
    Human Engineering and Risk Management Research Group, National Institute of Occupational Safety and Health
  • Ueda Takako Nakanishi-
    Department of Pharmacology, School of Medicine, Showa University
  • Ueda Toshihiko
    Department of Ophthalmology, School of Medicine, Showa University
  • Yasuhara Hajime
    Department of Pharmacology, School of Medicine, Showa University
  • Koide Ryohei
    Department of Ophthalmology, School of Medicine, Showa University

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Objectives: The aim of this study was to determine ultraviolet (UV) action spectra for cell killing of primary porcine lens epithelial cells (LECs) that can be used to establish guidelines for evaluation of the hazard of cataract due to UV exposure in the workplace. Methods: Primary porcine LECs were exposed to different doses (radiant exposure) of UV at 17 different wavelengths from 235 nm to 311 nm. At 2 days after exposure, cell viability was assessed by measuring crystal violet staining of the cells and lactate dehydrogenase release into the culture medium. The exposure dose required to kill 50% of cells (LD50) was determined from the dose-effect relationship obtained at each wavelength and was used to construct action spectra. Results: The action spectra had a broad minimum in the approximate range of 250–280 nm, indicating that UV is most hazardous to porcine LECs within this wavelength range. The spectra rose steeply at both longer and shorter wavelengths. These action spectra are consistent with the in vivo action spectra for opacities in the rabbit lens and for light scattering in the rat lens, taking the transmittance of the ocular media into account. Conclusions: These results will help to determine a UV hazard function for cataract formation, which can be used to draft guidelines for evaluation of the hazard of cataract due to UV exposure in the workplace.

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