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Simultaneous Determination of Glutathione, .GAMMA.-Glutamylcysteine and Cysteine in Commercial Yeast Extract by HPLC with Fluorimetric Detection.
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- KURODA Motonaka
- Food Research Laboratories, Ajinomoto Co. Ltd.
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- NAGABA Hiroki
- Food Research Laboratories, Ajinomoto Co. Ltd.
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- TSUBUKU Takako
- Food Research Laboratories, Ajinomoto Co. Ltd.
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- KAWAJIRI Hideo
- Food Research Laboratories, Ajinomoto Co. Ltd.
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- UEDA Yoichi
- Food Research Laboratories, Ajinomoto Co. Ltd.
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Description
A rapid and precise method for the simultaneous determination of glutathione (GSH), γ-glutamylcysteine and cysteine in commercial GSH-enriched yeast extract (GSH-YE) is described. Isocratic separation of the above compounds was performed using an aminopropyl silica column. Post-column derivatization with N-acridinylmaleimide (NAM) was performed followed by fluorimetric detection. This HPLC method could be applied for the determination of GSH, γ-glutamylcysteine and cysteine in commercial yeast extract. All of the commercial GSH-YE contained γ-glutamylcysteine (0.057-0.50 g/100 g) and cysteine (0.020-1.71 g/100 g).
Journal
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- Food Science and Technology International, Tokyo
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Food Science and Technology International, Tokyo 3 (3), 239-240, 1997
Japanese Society for Food Science and Technology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679431205504
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- NII Article ID
- 130000122836
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- COI
- 1:CAS:528:DyaK1cXhtVGhtbc%3D
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- ISSN
- 18813976
- 13417592
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed