Effects of Water Deficit on the Excitation

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  • Konishi Atsumi
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Omasa Kenji
    Graduate School of Agricultural and Life Sciences, The University of Tokyo

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Other Title
  • ホウレンソウ葉の水分欠乏に伴う励起
  • ホウレンソウ葉の水分欠乏に伴う励起-蛍光matrixへの影響
  • ホウレンソウ ヨウ ノ スイブン ケツボウ ニ トモナウ レイキ ケイコウ matrix エ ノ エイキョウ
  • -emission Matrix of Spinach Leaves
  • －蛍光matrixへの影響

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Abstract

Fluorescence spectrometry is a suitable method to non-destructively and remotely obtain information on the pigments, cell structure and physiological functions of plant leaves. In this study, the excitation-emission matrices of water-stressed spinach leaves in the dark were measured by a 3-D fluorescence spectrophotometer, and changes with time of these matrices were analyzed. Independently of the excitation wavelength, local maximum fluorescence intensities were captured at 450nm, 680nm, and 725 nm (F450, F680, and F725, respectively). F450/F680 and F450/F725 drastically changed with decreasing relative water content in the early stage of the water-deficit process. F450 hardly changed during the water-stressed treatment, which implied UV-defensible pigments were not accumulated in the dark. On the other hand, at an early stage of the water-deficit process, F680 and F725 appreciably decreased, and at that time, chlorophyll concentration increased, which implied chlorophyll contents in the measurement area were increased by the shrinkage of leaves, and fluorescence yield accordingly decreased. In the latter stage of the water-deficit process, F680 and F725 appreciably increased, which implied the photosynthetic reaction process was damaged by the extent of the water-deficit. As the excitation wavelength was shorter, F450/F680 and F450/F725 changed more extensively because F680 and F725 were smaller and F450 was larger. These results showed F450/F680 and F450/F725 excited by short-wavelength ultraviolet rays (for example, 330nm) were suitable for detecting changes of relative water content in spinach leaves.

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