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- Ando Hitoshi
- Department of Biophysics, Tsurumi University School of Dental Medicine
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- Inage Toshihiko
- Department of Anatomy, Nihon University School of Dentistry
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- Oida Shinichiro
- Department of Biochemistry and Molecular Biology, Tsurumi University School of Dental Medicine
書誌事項
- タイトル別名
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- Amelogenin in Frog Species, <i>Xenopus tropicalis</i>: A Gene Evolutionary Approach
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抄録
The structure and function of the amelogenin gene on a tooth germs in the frog Xenopus tropicalis, were examined. Using PCR, we observed an intensive band of amelogenin in maxillas with teeth but not in the toothless mandibles, and in situ hybridization revealed the restricted expression of amelogenin only in secreting ameloblasts in a tooth germs. Sequence analyses indicated that two types of amelogenin mRNA were alternatively spliced and derived from a single gene. They are referred to here as amelogenin mRNA-A and -B. In unique 5’-UTR, mRNA-A has a long exon 1, which is much longer than the same exon in other animals’ amelogenin, and both mRNA-A and -B possesses new exons, referred to here as exon 2a and 2b. The following protein coding region was divided into five exons, as in the case of another frog, Xenopus laevis, whose amelogenin sequence was closest to X. tropicalis, and the amelogenin of X. tropicalis may play a role in enamel formation, as in other animals. A pair of amelogenin mRNAs of X. tropicalis have the most primitive sequence among known mRNAs and, in particular, the extremely long exon 1, and the newly found exons 2a and 2b , may be key in exploring the archetype of amelogenin mRNA.
収録刊行物
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 22 (2), 189-194, 2013
硬組織再生生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679434285824
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- NII論文ID
- 130004480578
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- NII書誌ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL書誌ID
- 024714866
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可