Dental Pulp Cells with Multi-Potential for Differentiation to Odontoblast and Chondroblast

  • YAMASHITA Kikuji
    Department of Anatomy, School of Dentistry, University of Tokushima
  • James E. DENNIS
    Skeletal Research Center, Department of Biology, Case Western Reserve University
  • Donald P. LENNON
    Skeletal Research Center, Department of Biology, Case Western Reserve University
  • MORIMOTO Hiroyuki
    Department of Anatomy, School of Dentistry, University of Tokushima
  • KITAMURA Seiichiro
    Department of Anatomy, School of Dentistry, University of Tokushima
  • Arnold I CAPLAN
    Skeletal Research Center, Department of Biology, Case Western Reserve University

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The repair of dentin is thought to be maintained through differentiation of undefined precursor cells within the dental pulp to odontoblasts. To investigate the characteristics of these undefined precursor cells in dental pulp, mixed pulp cells and cloned cells were obtained from H-2Kb-ts A58 heterozygous transgenic mice. Differentiation of dental pulp precursor cells in vitro was assayed by RT-PCR analysis of gene expression of osteopontin, osteocalcin and dentin sialophosphoprotein (DSPP), and by von Kossa staining for mineralization. All assays were conducted on cultured mixed pulp cells and clonal cells PC1, PC8 and PC14. The results suggested that dental pulp cells could differentiate to odontoblasts in vitro. To test for chondrogenic potential, mixed pulp cells and clonal cells PC1, PC8 and PC14 were maintained in high density pellet cultures, and PC1 cells were stained with toluidine blue for glycosaminoglycans (GAG) content. To confirm the chondrocyte phenotype, the extracellular matrix of PC1 cells in pellet culture was stained with anti-type II collagen antibody. This study is the first to demonstrate that dental pulp stem cells present in the dental pulp have multi-potential to differentiate to both odontoblast and chondroblast.

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