Hydrogen Sulfide Suppresses Mineralized Nodule Formation by Osteoblastic ROS17/2.8 Cells

DOI Web Site 参考文献27件
  • Kimura Akemi
    Department of Oral Health Sciences, Nihon University School of Dentistry
  • Kawato Takayuki
    Department of Oral Health Sciences, Nihon University School of Dentistry Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry
  • Katono-Tani Tomoko
    Department of Oral Health Sciences, Nihon University School of Dentistry
  • Nakai Kumiko
    Nihon University Graduate School of Dentistry
  • Iwata Sakurako
    Nihon University Graduate School of Dentistry
  • Zhao Ning
    Department of Endodontics, School of Dentistry, Shandong University
  • Maeno Masao
    Department of Oral Health Sciences, Nihon University School of Dentistry Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry

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Hydrogen sulfide (H2S), which is the main component of the volatile sulfur compounds (VSCs) produced by putrefactive bacteria, plays a role in not only oral malodor, but also the initiation and progress of periodontitis. The loss of alveolar bone associated with periodontitis appears to be related to local factors that change the balance between bone formation and resorption. Previous studies have indicated that VSCs, including H2S, induce bone resorption by stimulating the differentiation and activation of osteoclasts; however, there is little information about the effect of VSCs on bone formation by osteoblasts. Therefore, we examined the effect of H2S on cell proliferation, alkaline phosphatase (ALPase) activity, non-collagenous extracellular matrix protein (ECMP) expression, and mineralized nodule formation using ROS17/2.8 cells as osteoblasts. Cells were cultured with 0 (control), 10-4, 10-3, or 10-2 M sodium hydrogen sulfide (NaHS; H2S donor). Mineralized nodule formation was detected by alizarin red staining. The expression of non-collagenous ECMP, including bone sialoprotein (BSP) and osteopontin (OPN), was examined at the mRNA and protein levels using real-time PCR and Western blotting, respectively. Cell proliferation was suppressed by the addition of 10-2 M NaHS, but was unaffected by 10-3 and 10-4 M NaHS. ALPase activity and the expression of BSP and OPN at the mRNA and protein levels were decreased when cells were cultured with 10-4 and/or 10-3 M NaHS. In addition, mineralized nodule formation was strongly inhibited by 10-4 and 10-3 M NaHS. These results suggest that H2S suppresses mineralized nodule formation by decreasing ALPase activity and the production of BSP and OPN by osteoblasts.

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