Low Level Fluoride Stimulates Epithelial-Mesenchymal Interaction in Oral Mucosa
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- He Dawei
- Department of Health Science, Division of Oral Health, Kanagawa Dental College
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- Ujjal K. Bhawal
- Department of Health Science, Division of Oral Health, Kanagawa Dental College Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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- Hamada Nobushiro
- Department of Infection Control, Division of Microbiology, Kanagawa Dental College
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- Kuboyama Noboru
- Department of Oral Molecular Pharmacology, Nihon University School of Dentistry at Matsudo
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- Abiko Yoshimitsu
- Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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- Arakawa Hirohisa
- Department of Health Science, Division of Oral Health, Kanagawa Dental College
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抄録
Oral mucosa consists of the superficial epithelium and the underlying lamina propria, and it functions as a barrier against exogenous substances. In development, interactions of stem/progenitor cells of the epithelium and mesenchyme are crucial to the morphogenesis of oral mucosa. Our previous work in low level fluoride-induced cell motility of epithelial cells has yielded important clues for periodontal physiology. This study focuses on a working concept of low level fluoride to provide a conducive oral environment for pivotal epithelial-mesenchymal interactions. Cultured human primary gingival epithelial cells treated with 50 μM NaF were investigated by DNA microarray. Quantitative real-time PCR and an in vivo experimental rat skin wound model were employed to confirm the findings obtained with the microarray analysis. Microarray data revealed that low level fluoride-treated human gingival epithelial cells elevated various biological processes. The key proliferation markers, FGF2 and FGF7, and mesenchymal marker, Twist1, expression was up-regulated and quantitative real-time PCR confirmed this observation. Our in vivo study revealed that low concentration of NaF increased FGF2, FGF7, and Twist1 protein expression in fluoride-treated skin wound tissues compared with controls. These results provide new information on low level fluoride-induced epithelial-mesenchymal interactions and may thus aid in the understanding of oral mucosa development.
収録刊行物
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 22 (1), 59-66, 2013
硬組織再生生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679435394560
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- NII論文ID
- 130004848411
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- NII書誌ID
- AA11074332
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- COI
- 1:CAS:528:DC%2BC3sXms1Kgt7o%3D
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- ISSN
- 1880828X
- 13417649
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- NDL書誌ID
- 024429628
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可