MicroRNA-29 Family Suppresses Mineralization in Dental Follicle Cells
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- Tomoki Risa
- Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
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- Ogura Naomi
- Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
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- Takahashi Kosuke
- Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
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- Ito Ko
- Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
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- Kondoh Toshirou
- Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
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Abstract
The dental follicle is an ectomesenchymal tissue surrounding developing tooth germ, and contains osteoblastic-lineage-committed stem/progenitor cells. MicroRNA (miRNAs) are small non-coding RNAs that regulate gene expression during stem cell growth, proliferation and differentiation. The aim of this study is to investigate the key regulators of miRNA during osteogenic differentiation in hDFC. We therefore analyzed miRNA expression profiles in hDFC during osteoblastic differentiation. Expression of miR-29a, -29b and 29c decreased in hDFC during osteogenic induction on microarray analysis. Real-time RT-PCR analysis also showed that the expression of miR-29 family members was significantly decreased in hDFC during osteogenic differentiation. The miR-29 family was predicted to target collagen type I alpha 1 and alpha 2 by in silico analysis. When miR-29s were transfected into hDFC, collagen type I production decreased. In addition, hDFC transfected with miR-29 mimics showed delayed mineralization when compared to hDFC transfected with negative control and nontrasfection culture. Our data suggest that miR-29 negatively regulates the osteogenic differentiation/mineralization of hDFC by targeting collagen type I.
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 24 (1), 23-28, 2015
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Keywords
Details 詳細情報について
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- CRID
- 1390282679435920512
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- NII Article ID
- 130004848438
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- NII Book ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL BIB ID
- 026057933
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed