Fabrication of a Fluidic Channel Integrated with Electrodes for Cell-based Chemical Sensor
-
- Misawa Nobuo
- EIIRIS, Toyohashi University of Technology
-
- Lee Hyunjae
- ICG, Ishida & Sawada Lab. Toyohashi University of Technology
-
- Sawada Kazuaki
- EIIRIS, Toyohashi University of Technology ICG, Ishida & Sawada Lab. Toyohashi University of Technology Core Research for Evolutional Science and Technology, JST
Bibliographic Information
- Other Title
-
- 細胞を用いた多チャンネル化学量センサのための電極一体型細胞アレイ化流路の作製
- サイボウ オ モチイタ タチャンネル カガクリョウ センサ ノ タメ ノ デンキョク イッタイガタ サイボウ アレイカ リュウロ ノ サクセイ
Search this article
Abstract
This paper shows the fabrication of a fluidic device for measuring of membrane potential changes of multiple cells. The device is composed of poly(methyl methacrylate) (PMMA) and a silicon substrate that has needle-shaped structures for electrodes. The electrodes are for “two-electrode voltage clamping method” that can measure ion flows across cell membrane using an amplifier and two electrodes inserted into a cell. In this device, Xenopus laevis oocytes can be arrayed automatically in the fluidic channel and penetrated by electrode structures at the same time. To construct the fluidic device integrated with electrodes, we simulated and calculated the fluid behavior for oocytes array and investigated the flow rate for electrodes insertion into oocytes.
Journal
-
- IEEJ Transactions on Sensors and Micromachines
-
IEEJ Transactions on Sensors and Micromachines 133 (5), 135-138, 2013
The Institute of Electrical Engineers of Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679437785472
-
- NII Article ID
- 10031167008
-
- NII Book ID
- AN1052634X
-
- ISSN
- 13475525
- 13418939
-
- NDL BIB ID
- 024670261
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed