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QCM Sensor Using Membrane Proteins of Cell-derived Liposomes

  • Yamanaka Makoto
    Research Center for Bio-microsensing Technology, Kyushu Institute of Technology
  • Yasuda Takashi
    Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology

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  • 細胞由来リポソーム上の膜タンパク質を利用したQCMセンサ

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Abstract

This paper presents a novel QCM (quartz crystal microbalance) sensor for detecting interactions between membrane proteins and their ligands using liposomes derived from human cells. First, liposomes having BCR (B-cell receptor) were derived directly from cell membranes by stimulating human lymphocyte cells, Ramos, with NaB (sodium butyrate). Next, the liposomes were separated from the cells by filtration and micronized by sonication. Then, the liposomes were immobilized on the surface of a QCM sensor chip using cell-anchoring molecules. Several experiments demonstrated that ligand detection is achieved by frequency reduction with application of the antibody against BCR.

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