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This paper presents a novel QCM (quartz crystal microbalance) sensor for detecting interactions between membrane proteins and their ligands using liposomes derived from human cells. First, liposomes having BCR (B-cell receptor) were derived directly from cell membranes by stimulating human lymphocyte cells, Ramos, with NaB (sodium butyrate). Next, the liposomes were separated from the cells by filtration and micronized by sonication. Then, the liposomes were immobilized on the surface of a QCM sensor chip using cell-anchoring molecules. Several experiments demonstrated that ligand detection is achieved by frequency reduction with application of the antibody against BCR.
- IEEJ Transactions on Sensors and Micromachines
IEEJ Transactions on Sensors and Micromachines 133 (5), 155-156, 2013
The Institute of Electrical Engineers of Japan