Sensitivity Improvement of Dielectrophoretic Impedance Measurement by Bacteria Concentration using Negative Dielectrophoresis

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  • Inoue Yuki
    Faculty of Information Science and Electrical Engineering, Kyushu University
  • Nakano Michihiko
    Faculty of Information Science and Electrical Engineering, Kyushu University
  • Suehiro Junya
    Faculty of Information Science and Electrical Engineering, Kyushu University

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  • 負の誘電泳動力による細菌濃縮を利用した誘電泳動インピーダンス計測法の高感度化
  • マケ ノ ユウデン エイドウリョク ニ ヨル サイキン ノウシュク オ リヨウ シタ ユウデン エイドウ インピーダンス ケイソクホウ ノ コウカンドカ

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Abstract

Dielectrophoretic impedance measurement (DEPIM) is a method to detect bacteria in an aqueous solution. DEPIM consists of two simultaneous processes: dielectrophoretic trapping of the target bacteria onto a microelectrode and measurement of impedance change caused by trapping bacteria. In this study, a new bacterial concentration process was combined with DEPIM to improve its sensitivity. The concentration process was based on negative dielectrophoresis of bacteria caused by specific configuration of parallel electrodes. A ditched dielectric thin layer deposited on one of the parallel electrodes causes non-uniform electric field resulting in occurring negative dielectrophoresis. Two electrode configurations, one is for the bacteria concentration and the other is for DEPIM, were fabricated in a microchannel. As a result, the detection sensitivity of DEPIM increased about 1.6 times by combining the concentration.

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