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Hydrocarbon Degradation and Bacterial Community Responses During Remediation of Sediment Artificially Contaminated with Heavy Oil

  • N. NUÑAL SHARON
    Institute of Fish Processing Technology, College of Fisheries and Ocean Sciences, University of the Philippines Visayas
  • SANTANDER-DE LEON SHEILA MAE S.
    Institute of Marine Fisheries and Oceanology, College of Fisheries and Ocean Sciences, University of the Philippines Visayas
  • HONGYI WEI
    Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University
  • REGAL ADEL AMER
    National Institute of Oceanography and Fisheries
  • YOSHIKAWA TAKESHI
    Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University
  • OKUNISHI SUGURU
    Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University
  • MAEDA HIROTO
    Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University

Abstract

<p> Natural biodegradation of heavy oil in the marine environment can be accelerated by the addition of nutrients or seeding of pre-selected microorganisms. In this study, a microcosm experiment was conducted to investigate the effects of inorganic nutrient supplementation (biostimulation) and bacterial consortium amendment (bioaugmentation) on the natural degradative processes of artificially contaminated sediment. Our results revealed that the addition of nutrients had greater effect on remediation than the addition of bacterial cells. Supplementation of inorganic nutrients promoted and sustained the growth of oil-degrading and heterotrophic bacteria throughout the experimental period. Highest reduction in the total petroleum hydrocarbons, and of their components, n-alkanes, polycylic aromatic hydrocarbons (PAHs) and alkyl PAHs, were obtained in the biostimulated microcosms. Changes in the bacterial community were monitored by the PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) method targeting the 16S rDNA gene. Results revealed different responses of the bacterial community to the addition of heavy oil and remediation agents. Shifts in the bacterial communities in the seawater were more dynamic than in the sediment. Results of this study showed that addition of remediation agents significantly enhanced the natural biodegradation of heavy oil in a sediment-seawater microcosm trial.</p>

Journal

  • Biocontrol Science

    Biocontrol Science 22 (4), 187-203, 2017

    The Society for Antibacterial and Antifungal Agents, Japan

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