Evaluation of a New Most-Probable-Number (MPN) Dilution Plate Method for the Enumeration of Escherichia coli in Water Samples

DOI PubMed 34 References Open Access
  • KODAKA HIDEMASA
    Nissui Pharmaceutical Co. Ltd., Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • SAITO MIKAKO
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • MATSUOKA HIDEAKI
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

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Description

The purpose of this study was to evaluate the most-probable-number dilution plate (MPN plate) method developed for the enumeration of Escherichia coli in water samples. Sterilized water was inoculated with E. coli ATCC 11775 to give between 2-1600 MPN/100ml. The MPN was determined for both the MPN plate and 5-tube methods from the MPN table. The average of the natural logarithm (ln) MPN with standard deviations in 95 samples was 4.26 +/- 1.48 by the 5-tube-method and 4.18 +/- 1.45 by the MPN plate method. The correlation coefficient was 0.96. These results were not significantly different according to the paired t-test (p>0.05).

Journal

  • Biocontrol Science

    Biocontrol Science 14 (3), 123-126, 2009

    The Society for Antibacterial and Antifungal Agents, Japan

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Details 詳細情報について

  • CRID
    1390282679441364480
  • NII Article ID
    10025624363
  • NII Book ID
    AA11169621
  • DOI
    10.4265/bio.14.123
  • COI
    1:STN:280:DC%2BD1MnltlCqtQ%3D%3D
  • ISSN
    18840205
    13424815
  • PubMed
    19785286
  • Text Lang
    en
  • Article Type
    journal article
  • Data Source
    • JaLC
    • Crossref
    • PubMed
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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