Development of a PCR-based Marker Set for Identifying Koshihikari Niigata BLs, Near-isogenic Lines Harboring Rice Blast Resistance Genes

Nakamura Sumiko, Suzuki Keitaro, Ban Yoshiyuki, Nishikawa Tsuneo, Tokunaga Kunio, Ohtsubo Ken’ichi

doi:10.1270/jsbbr.8.79

Blast disease is one of the most devastating diseases of rice. To minimize losses of production due to the disease, the use of multilines, which are groups of near-isogenic rice lines harboring various resistance genes in a common background, is considered to be a useful strategy for disease control. Blast resistant-multilines derived from Koshihikari and some other elite Japanese cultivars have been developed. In the present study, we generated five STS-based DNA markers for identifying blast-resistant Koshihikari Niigata BLs harboring either of Pia, Pii or Pita-2. It was revealed that each of these markers produced a PCR product mostly with DNAs from a Koshihikari BL and other cultivars harboring the respective resistance gene, unlike with DNAs from blast-susceptible Koshihikari and other Japanese representative rice cultivars. Based on homology search of the sequence of the markers against published Nipponbare genome sequence data, it was found that each of these markers was positioned in a chromosomal region where the respective blast gene had been reported to be located. These DNA markers, especially if the multiplex PCR procedure is used, may enable to achieve efficient discrimination of Niigata BLs 1, 2 and 3 from one another, from other Niigata BLs and from other Japanese rice cultivars.<br>

Development of a PCR-based Marker Set for Identifying Koshihikari Niigata BLs, Near-isogenic Lines Harboring Rice Blast Resistance Genes

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