イネ縞葉枯病抵抗性遺伝子Stvb-iを検出するSCARマーカー

DOI 日本農学文献記事索引 Web Site Web Site 被引用文献6件 参考文献14件

書誌事項

タイトル別名
  • イネ縞葉枯病抵抗性遺伝子ｓｔｖｂ‐ｉを検出するＳＣＡＲマーカー
  • SCAR Marker for Selection of the Rice Stripe Resistance Gene <I>Stvb-i</I>
  • イネジマ ハガレビョウ テイコウセイ イデンシ Stvb i オ ケンシュツ スル SCAR マーカー

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説明

A stripe resistance gene, Stvb-i, was introduced into japonica cultivars from an indica cultivar Modan in Japan. A set of primers for PCR assay was designed based on the sequence information of a RFLP marker ST10 which is linked by 0.0 cM to the Stvb-i gene. This set of primers, which generate a SCAR (sequence characterized amplified region) marker, was referred to as “ST10 primers” . In PCR with the ST10 primers, a 727-bp DNA fragment was amplified specifically from all the tested rice plants harboring the Stvb-i gene derived from Modan: resistant cultivars, lines and F1 individuals from a cross of the susceptible parent ‘Koganebare’/resistant parent ‘Tsukinohikari’. On the other hand, the fragment was not amplified in susceptible cultivars. In 120 of the F2 individuals derived from the susceptible parent ‘Koshihikari’/resistant parent ‘Asanohikari’, the resistant homozygous and heterozygous individuals showed a positive reaction in the PCR assay with the ST10 primers. The 727-bp fragment was identified as a part of the ST10 fragment (745 bp) by sequence analysis. In foreign cultivars, the ST10 primers amplified the 727-bp fragment in 3 out of 9 tested resistant cultivars that have been reported to harbor the same resistance gene, Stvb-i. The three cultivars were Modan, IR8 and Mudgo. The 727-bp fragment was not amplified in a foreign cultivar ‘Zenith’and Japanese upland cultivars harboring other resistance genes, Stva and Stvb. The SCAR marker can be used in the markeraided selection of stripe resistant individuals carrying the Stvb-i gene derived from Modan. Therefore, screening for stripe resistance can now be conducted independently of the bioassay with vector insects.

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