高出力レーザーの低出力照射がラット骨髄細胞三次元培養の石灰化に及ぼす影響

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  • Effects on Mineralization by Low-level Irradiation of Hard Laser in Three-dimensional Cultured Rat Bone Marrow Cells

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Recently, the photobioactive reaction (PAR) effect which causes proliferation and differentiation of cells by using a high-power laser with a low output has been developed, and is used by lowering the output and shortening the irradiation time for low level-laser treatment (LLLT) to promote healing. However, there is little research on the use of low-output irradiation of high-power laser for cultured osteoblast cells.<BR>This study evaluated the effect of low-level irradiation of hard laser, which uses a CO2 laser and a semiconductor laser, on the mineralization of cultured rat bone marrow cells. We used a three-dimensional culture system of rat bone marrow cells to simulate the in vivo condition.<BR>A significant difference of calcium amount under 2.6W laser irradiation was found. However, the semiconductor laser did not lead to any increase of calcium amount.<BR>This result shows that CO2 laser irradiation has the effect of promoting calcification by osteoblasts, and optimal energy seems to exist around 2.6W. However, there was no effect of promoting calcification by semiconductor laser irradiation.<BR>In this study, laser did not promote calcification without the addition of dexamethasone, which accelerates osteoblast differentiation. This result shows that laser did not promote the differentiation to osteoblasts, and it was thought that laser promoted calcification of differentiated osteoblasts. The highest expression of HSP27 was shown under 2.6W of CO2 laser irradiation, which yielded the highest amount of calcium. It seems possible that the cascade of HSP27 involved the calcification of osteoblasts, which is stimulated by laser irradiation.

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