The Effect of Nd:YAG Laser Irradiation on Human Gingival Fibroblasts
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- YOSHIHASHI Naoya
- Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University
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- MURAKASHI Etsuko
- Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University
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- TAKEUCHI-IGARASHI Hiroko
- Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University
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- NUMABE Yukihiro
- Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University
Bibliographic Information
- Other Title
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- Nd:YAGレーザーがヒト歯肉線維芽細胞に与える影響について
- The Effect of Nd:YAG Laser Irradiation on Human Gingival Fibroblasts—A Study of the Irradiation Output and Distance
- —A Study of the Irradiation Output and Distance—
- ―照射出力および照射距離に関する検討―
Description
This study was performed in order to investigate the effects of Nd:YAG laser irradiation on human gingival fibroblasts (Gin-1), as represented by cell proliferation activity rate and TGF-β1 and Type I collagen production rate, as well as HSP47 expression.<br>Gin-1 cells were irradiated for 10 seconds at laser outputs of 2W (5pps/400mJ, 100pps/20mJ) and 7.2W (40pps/180mJ, 90pps/80mJ), at irradiation distances of 10mm or 20mm. Culture supernatant was recovered after 3 and 5 days of culture following laser irradiation and investigated for cell proliferation rate (modified MTT), and TGF-β1 and Type I collagen production rate (ELISA method). Gin-1 was also subjected to immunofluorescent staining after 3 hours of culture following laser irradiation, and observed for HSP47 expression by confocal laser scanning microscopy.<br>On the 5th day of culture, both of the groups irradiated by 2W at 10mm exhibited significantly higher cell proliferation rates than controls, and on the 3rd day of culture the group irradiated by 7.2W (90pps/80mJ) at 10mm exhibited significantly higher TGF-β1 production rates than controls. Among the 10mm irradiation groups on the 3rd day, both of the 7.2W groups exhibited significantly higher Type I collagen production rates than controls. Intracytoplasmic HSP47 expression was found in all groups.<br>Thus, high-output laser irradiation with an increased irradiation distance exerts a cell activating effect without harmful heating, similar to that of low-output laser irradiation.
Journal
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- Journal of Japanese Society for Laser Dentistry
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Journal of Japanese Society for Laser Dentistry 24 (2), 72-82, 2013
Japanese Society for Laser Dentistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282679452243328
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- NII Article ID
- 130004565026
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- ISSN
- 21856702
- 09177450
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed
