逆相ＴＬＣ，分光蛍光光度計及び蛍光検出器付きＨＰＬＣによる局方ガーゼ中の蛍光増白剤の分析

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タイトル別名
  • Analysis of Fluorescent Whitening Agents in the Japanese Pharmacopoeia Absorbent Gauze Using Reversed Phase TLC, Spectrofluorophotometer and HPLC with Fluorescence Detector.
  • ギャクソウ TLC ブンコウ ケイコウ コウドケイ オヨビ ケイコウ ケンシュ

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抄録

The purity test of the Japanese Pharmacopoeia Absorbent Gauze for fluorescent whitening agents is regulated to perform by a method of irradiating the gauze with ultraviolet rays in a dark place. This method has been reported to have a difficulty in detecting the existence of fluorescent whitening agents in several cases. We, therefore, tried to establish a method to examine the existence of fluorescent whitening agents using physical and chemical methodologies. After cutting the Japanese Pharmacopoeia Absorbent Gauze samples in small pieces, fluorescent whitening agents were extracted by 30% pyridine solution at 100°C for 30 min. We analysed the fluorescent agents using reversed phase TLC, spectrofluorophotometer and HPLC with a fluorescence detector using 8 standard fluorescent whitening agents as controls. Conditions of reversed phase TLC : plate, RP-18W (Merck) ; solvent system, A) acetonitrile-water (1 : 4), B) acetonitrile-methanol-water (3 : 3 : 10), C) methyl ethyl ketone-methanol-water (1 : 1 : 1). Conditions of HPLC with fluorescence detection : column, Wakosil 5C18 (i.d. 4.6×150 mm) ; mobile phase, 0.01M ammonium acetate-acetonitrile (7 : 3) ; flow rate, 1.0 ml/min ; injection volume, 20 μl ; detections, fluorescence detector (excitation 345 nm and emission 435 nm).

収録刊行物

  • 衛生化学

    衛生化学 44 (5), 364-370, 1998

    公益社団法人 日本薬学会

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