Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection
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- Yamamoto Hidetaka
- Department of Microbiology, Suzuka University of Medical Science
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- Ura Yukari
- Department of Microbiology, Suzuka University of Medical Science
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- Tanemura Miho
- Department of Microbiology, Suzuka University of Medical Science
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- Koyama Aoi
- Department of Microbiology, Suzuka University of Medical Science
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- Takano Sayaka
- Department of Microbiology, Suzuka University of Medical Science
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- Uematsu Jun
- Department of Microbiology, Suzuka University of Medical Science
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- Kawano Mitsuo
- Department of Microbiology, Mie University Graduate School of Medicine
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- Tsurudome Masato
- Department of Microbiology, Mie University Graduate School of Medicine
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- O'Brien Myles
- Graduate School of Mie Prefectural College of Nursing
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- Komada Hiroshi
- Department of Microbiology, Graduate School of Clinical Nutrition, Suzuka University of Medical Science
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Abstract
Lactoferrin (Lf) is a multifunctional protein that has inhibitory activity against microorganisms. In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, virus RNA synthesis was only slightly inhibited by Lf. In addition, indirect immunofluorescence study showed that virus protein syntheses were not completely inhibited by Lf. Using a recombinant, green fluorescence protein-expressing hPIV-2 (rghPIV-2), it was found that virus entry into cells were considerably inhibited by Lf, but cell-to-cell spread was not inhibited. The number of viruses produced by the cells were determined, and it was found that Lf reduced the number of released viruses to about 1/300 compared with that of positive control. Lf bound to cell surface within 10 min at early phase of infection, it assembled gradually, and many aggregates were observed at 120 min. These results indicated that Lf considerably inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented hPIV-2 infection.
Journal
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- Journal of Health Science
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Journal of Health Science 56 (5), 613-617, 2010
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679473801728
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- NII Article ID
- 130000425098
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- NII Book ID
- AA11316464
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- COI
- 1:CAS:528:DC%2BC3cXhtlags7vK
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- ISSN
- 13475207
- 13449702
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- NDL BIB ID
- 10825254
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed