Prazosin stimulates the release of hepatic triacylglycerol lipase caused from primary-culture rat hepatocytes

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  • Nakamura Tetsuya
    Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
  • Kerakawati Rie
    Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
  • Morita Tetsuo
    Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University

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  • Prazosin Stimulates the Release of Hepatic Triacylglycerole Lipase Caused from Primary-culture Rat Hepatocytes

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Prazosin is an alpha 1 adrenoceptor antagonist, and it is used as an antihypertensive agent. The effects of prazosin on the activity of hepatic triacylglycerole lipase (HTGL) are not fully understood. In this study, we demonstrated that prazosin stimulates the release of HTGL activity from primary cultures of rat hepatocytes in a time- and dose-dependent manner. U-73122, a phopholipase C (PLC) inhibitor, suppresses prazosin's stimulation of the release of HTGL activity. Moreover, prazosin stimulated the increase of PLC activity in the hepatocytes in a time- and dose-dependent manner. In addition, the prazosin-stimulated release of HTGL activity was reduced by Quin2/AM (an intracellular Ca2+-chelator), W-7 (a Calmodulin inhibitor), and KN-93 [an inhibitor of Ca2+/Calmodulin dependent protein kinase (CaMK)-II]. These results suggest that the prazosin-stimulated release of HTGL activity is partly due to the activation of CaMK-II that is associated with the elevation of PLC activity in the hepatocytes.

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