Histone Methyltransferase PR-Set7 and Histone Variant H2A.Z, Induced during Hepatocarcinogenesis, Repress the Promoter Activity of the Tumor Marker Gene and the Ras-Induced Colony Formation Activity

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  • Hashizume Hiroshi
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Gomita Urara
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Imagawa Masayoshi
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Osada Shigehiro
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University

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Genetic and epigenetic studies are required to understand molecular mechanisms of carcinogenesis and tumorigenesis. Although alterations of DNA methylation and histone modification profiles are observed in cancer cells, the knowledge of the epigenetic regulatory factors involved in carcinogenesis is insufficient. In this study, we showed that the histone variant, H2a.z, and the histone methyltransferase, Pr-set7/Set8/KMT5a, were up-regulated during chemically induced hepatocarcinogenesis. During this process, the glutathione S-transferase placental form (GST-P), which is completely repressed in normal liver, is strongly induced and is therefore an excellent tumor marker. Reporter analysis performed using the regulatory region of the Gst-p gene revealed that H2A.Z and PR-SET7 repressed Gst-p promoter activity. The enhancer element responsible for hepatocarcinogenic-specific gene expression was required for repression by H2A.Z, and the negative regulation by PR-SET7 mediated the regulatory element but not the enhancer. Furthermore, we examined the effects of overexpression of H2A.Z and PR-SET7 on the colony formation activity of mouse NIH-3T3 cells grown on a soft agarose medium. Colony formation based on anchoring independent cell growth is a feature of malignant transformed cells. These factors did not exhibit colony formation activity but repression of the ras oncogene induced this activity. PR-SET7 suppressed RASval12-mediated colony formation through methyltransferase activity. These results suggest that H2a.z and Pr-set7 that are induced during hepatocarcinogenesis may function as carcinogenesis suppressors.

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