Studies of an in vitro alternative method to the draize rabbit eye irritation test. Comparison of hemolysis of erythrocytes and neutral red uptake in culture cell.

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Other Title
  • 眼刺激性試験の代替法の研究　溶血法と培養細胞を用いた方法の比較検討
  • Comparison of Hemolysis of Erythrocytes and Neutral Red Uptake in Culture Cell
  • 溶血法と培養細胞を用いた方法の比較検討

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Description

Surface active agents (surfactants) were tested for hemolysis of rat erythrocytes and neutral red uptake using rabbit corneal cell (SIRC). The effects of hemolysis were manifested as the concentration of surfactants at 50% hemolysis of erythrocytes (HC-50 values). The effects of NR-uptake were manifested as the concentration of surfactants at 50% NR-uptake compared with untreated controls (NR-50 values). These results were compared with in vivo eye irritation score of surfactants obtained by Draize test.<br>Good correlations were seen between these inverse numbers of two results (HC-50 values and NR-50 values) and the Draize scores. The results suggest that both the hemolysis assay and the neutral red assay may be useful as an alternative to the Draize test as an in vitro method for detecting eye irritation.

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Details 詳細情報について

  • CRID
    1390282679477453696
  • NII Article ID
    130004188630
  • DOI
    10.5107/sccj.23.272
  • ISSN
    18844146
    03875253
    http://id.crossref.org/issn/03875253
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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