Simple and Accurate HPLC Method for Simultaneous Determination of UV Absorbers in Cosmetics

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  • 液体クロマトグラフィーによる化粧品中の紫外線吸収剤の簡易一斉分析法
  • エキタイ クロマトグラフィー ニ ヨル ケショウヒン チュウ ノ シガイセン キュウシュウザイ ノ カンイ イッセイ ブンセキホウ

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For quantitative analysis of UV absorber in sunscreen products, a simultaneous determination with high performance liquid chromatography (HPLC) is useful. Many studies on simultaneous determination have been reported. However, most authors adopt a gradient method that is not appropriate for routine analysis, due to the long time needed to stabilize the system after returning the solvent condition to the default before the next sample injection. Herein, we propose an isocratic method, using HPLC on a C30 column. We apply the approach in the simultaneous determination of seven UV absorbers in cosmetic products, and demonstrate that it is an accurate, simple, and effective detection method.

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