Pilot-Plant Study of Two-Step Continuous Conversion of Cane Molasses by Yeast Invertase Immobilized in Alginate Gel in L-Glutamic Acid Fermentation.

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  • グルタミン酸発酵原料のケインモラセスに含まれるしょ糖の転化に用いるアルギン酸固定化インベルターゼの反応器特性
  • グルタミンサン ハッコウ ゲンリョウ ノ ケインモラセス ニ フクマレル ショ

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Abstract

Use of converted sugar in L-glutamuc acid fermentation increases productivity. Cane molasses was used instead of sucrose as the main carbon source in a pilot-plant study done with yeast invertase trapped in Na-alginate and a pair of agitation-type reactors each with a capacity of 35-1. Two-step continuous reaction gave a conversion ratio of 95% when the initial sugar concentration was 550g/l, the enzyme packing ratio was 30%, the mean retention time was 7h, and the temperature was 50°C. Problems did not occur on this increased scale. The retention time that would give a conversion ratio of 95% in reactors of this size calculated with the Michaelis-Menten equation from experimental results obtained with a 100-ml reactor agreed with the experimental value obtained with the 35-1 reactors.

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 67 (11), 1575-1581, 1993

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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