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- HANADA Koichi
- Research Institute, Meiji Sugar Mfg. Co., Ltd.
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- HIROSE Akira
- Higashi Nippon Sugar Mfg. Co., Ltd.
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- NINOMIYA Eizi
- Research Institute, Meiji Sugar Mfg. Co., Ltd.
Bibliographic Information
- Other Title
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- デキストラナーゼ精製酵素の諸性質
- デキストラナーゼ セイセイ コウソ ノ ショ セイシツ Aspergillus ustus 237 ノ セイサン スル デキストラナーゼ ニ カンスル ケンキュウ 2
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Abstract
Dextranase which was produced by Aspergillus ustus 237 was purified to approximately 1000 folds by means of ammonium sulfate fractionation, DEAF-cellulose column. chromatography and Bio-Gel P-150 column chromatography.<br> Purified enzyme was appeared to be homogenous in disc-electrophoretic analysis and its molecular weight was estimated to be approximately 35, 000 by gel-filtration on Bio-Gel P-150.<br> Optimum pH and temperature for the enzyme reaction were pH 5.7 and 50°C, respectively. The enzyme was stable in the pH range of 5.5 to 8.5 and also at the temperatures less than 40°C, while more or less 50% of the enzyme activities were inactivated by heating to 50°C for 15 minutes at pH 7.0. However, it was found that the enzyme showed the remarked heat-tolerance in the presence of some cations, such as Cr3+ and Al3+. The dextranase activity was inhibited completely by Hg2+, Ag+ and permanganate and was scarcely by chelating agents reductants.
Journal
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- Nippon Nōgeikagaku Kaishi
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Nippon Nōgeikagaku Kaishi 48 (1), 21-26, 1974
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282679482408576
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- NII Article ID
- 130001223404
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- NII Book ID
- AN00196191
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- COI
- 1:CAS:528:DyaE2MXhtVyjsbc%3D
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- ISSN
- 18836844
- 00021407
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- NDL BIB ID
- 7622934
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed