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- OKADA Sanae
- Nodai Research Institute
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- UCHIMURA Tai
- Faculty of Agricultural Chemistry, Tokyo University of Agriculture
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- OHARA Naohiro
- Faculty of Agricultural Chemistry, Tokyo University of Agriculture
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- KOZAKI Michio
- Faculty of Agricultural Chemistry, Tokyo University of Agriculture
Bibliographic Information
- Other Title
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- 寒天平板による乳酸菌の糖類発酵性新試験法
- カンテン ヘイバン ニヨル ニュウサンキン ノ トウルイ ハッコウセイ シン
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Abstract
The sugar fermentation test is an important criteria in identification of lactic acid bacteria. Usually, the sugar fermentation is detected by acidformation from a sugar given in growth media. In the ordinary method, acid-formation is indicated by the value of titration of the media by alkali-solution or by the color-change dye (i.e. bromcresol purple) previously added to the media.<br> In the present paper, we described a new method using an agar-plate in the sugar fermentation test. An agar-plate containing one sugar as the sole carbon source, yeast extract, peptone, inorganic salts and CaCO3 as the acid indicator was prepared in a 90mm diameter petridish. The agarplate was covered with sterile 1.5% agar. Test strains of lactic acid bacteria were inoculated on the agar-plate by stabbing.<br> The sugar fermentation reaction was easily detected by observing the incubated agar-plates. In positive reaction, a clear zone of dissolved CaCO3 by the acid produced was observed around the stab line on the agar-plate; whereas in negative reaction, the clear zone was not observed. A maximum of 20 lactic acid bacteria strains can be tested on an agar-plate prepared in a 90mm petridish.
Journal
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- Nippon Nōgeikagaku Kaishi
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Nippon Nōgeikagaku Kaishi 57 (3), 227-230, 1983
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282679483418240
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- NII Article ID
- 130001229236
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- NII Book ID
- AN00196191
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- COI
- 1:CAS:528:DyaL3sXktVamtr4%3D
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- ISSN
- 18836844
- 00021407
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- NDL BIB ID
- 2585964
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed