固定化酵素反応のみかけの最大反応速度およびＭｉｃｈａｅｌｉｓ定数の基質濃度依存性

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  • Substrate concentration dependence of apparent maximum reaction rate and Michaelis constant observed in immobilized enzyme reactions.
  • コテイカ コウソ ハンノウ ノ ミカケ ノ サイダイ ハンノウ ソクド オヨビ

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説明

Expressions for apparent kinetic parameters V appm ane K appmobserved in immobilized enzyme reactions with diffusional and electrostatic effects are proposed on the basis of the equation of a tangent to the curve of a Sb/υ versus Sb plot. The validity of these expressions is shown by comparing the expression for K appm with two approximate expressions proposed by other workers for the reaction catalyzed by an enzyme immobilized on the external surface of a nonporous support. Calculation showed that the effect of substrate concentration on V appm and K appm is basically different for systems where an enzyme is immobilized on the external and pore surfaces respectively of supports.

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